Abstract
Introduction: Renal ischemia-reperfusion (RIR) injury is one of the main causes of acute kidney failure.
Objectives: The purpose of this study was to assess the effects of vitamin E, as well as a combination of vitamin E and sodium selenite (Se), on a rat model of RIR injury.
Materials and Methods: Current study was a laboratory experimental investigation using a post-test only control group desgin. A total of thirty-two adult male Sprague Dawley rats were divided into four equal groups: group 1 (control), group 2 [(IR; ischemia-reperfusion) + 0.25 mL saline)], group 3 (IR+1 mg/kg sodium Se and 100 mg/kg vitamin E), and group 4 (IR+100 mg/kg vitamin E). RIR injury was initiated by clamping the right and left pedicles for a duration of 45 minutes, followed by a 24-hour period of reperfusion. The intraperitoneal administration of daily therapy commenced 12 days prior to the development of RIR.
Results: Ischemia-reperfusion injury resulted in a considerable elevation in serum levels of urea, creatinine, and malondialdehyde (MDA), as well as enhanced serum myeloperoxidase (MPO) activity and renal MDA levels. Nevertheless, RIR markedly reduced the concentration of glutathione (GSH) in the serum, as well as the enzymatic activities of glutathione peroxidase (GPX) and paraoxonase 1 (PON1) in the serum. Additionally, RIR decreased the enzymatic activities of GPX and catalase (CAT) in the kidneys. In RIR animals, sodium Se plus vitamin E significantly improved renal function parameters, MDA content, GSH, and GPX activity in the kidneys.
Conclusion: Our findings showed that the effects of sodium Se and vitamin E on reducing oxidative stress and inflammatory markers and improving renal function biomarkers were comparable to those of vitamin E alone.