Submitted: 10 Aug 2020
Accepted: 04 Dec 2020
ePublished: 23 Jan 2021
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J Renal Inj Prev. 2021;10(3): e24.
doi: 10.34172/jrip.2021.24
  Abstract View: 55
  PDF Download: 38


D-limonene in diabetic rats

Shahrokh Bagheri 1,2 ORCID logo, Mostafa Moradi Sarabi 3 ORCID logo, Mohammadreza Gholami 4 ORCID logo, Vahideh Assadollahi 5 ORCID logo, Reza Mohammadrezaei Khorramabadi 2 ORCID logo, Forouzan Hadipour Moradi 1, Hassan Ahmadvand 3 * ORCID logo

1 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
3 Department of Biochemistry and Genetics, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
4 Medical Technology Research Center, Institute of Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran
5 Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
*Corresponding author: Hassan Ahmadvand, Email: ahmadvand.h@lums.ac.ir, hassan_a46@yahoo.com


Introduction: Diabetes mellitus (DM) is a multi-factorial condition associated with oxidative stress. Limonene, as a plant-derived antioxidant, can be used for treating DM.

Objectives: An investigation on antioxidant effects in diabetic rats exposed to D-limonene.

Materials and Methods: Sixty male Wistar rats were categorized into six groups as follows: control (healthy rats), diabetic control (untreated diabetic rats), sham glibenclamide, diabetic glibenclamide, sham limonene, and finally diabetic limonene. Alloxan (100 mg/dL) was infused intraperitoneally to induce type 1 diabetes in rats. Rats in certain groups were given limonene (100 mg/dL) and glibenclamide (10 mg/dL) orally for 8 weeks. Subsequently, animals were killed, and their kidneys were removed. Serum levels of biochemical factors (including serum creatinine, urea, and glucose) were determined, and factors such as nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), and myeloperoxidase (MPO) were measured in kidney tissue homogenate. The gene expression and enzymatic activity of glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) in the kidney were measured by real-time polymerase chain reaction (real-time PCR) and spectrophotometry, respectively.

Results: Limonene treatment significantly decreased serum glucose, creatinine, and urea. Additionally, MDA, MPO, and NO significantly decreased while GSH increased after treatment with limonene. Real-time RT-PCR showed significant elevation (P<0.05) in mRNA levels of GPx, CAT, and SOD in the limonene-treated compared with the diabetic control group.

Conclusion: Our results demonstrated that limonene as an herbal antioxidant had better effects on antioxidant markers compared to glibenclamide in rat models of diabetes.

Keywords: Diabetes, Limonene, Oxidative stress, Antioxidant enzymes, Gene expression

Implication for health policy/practice/research/medical education:

Our study exhibited that D-limonene could ameliorate serum glucose, creatinine, urea, the activities of antioxidant enzymes (GPx, CAT and SOD), the levels of MDA, GSH, NO, and MDA activity in the diabetic treated group.

Please cite this paper as: Bagheri S, Moradi Sarabi M, Gholami M, Assadollahi V, Mohammadrezaei Khorramabadi R, Hadipour Moradi F, Ahmadvand H. D-limonene in diabetic rats. J Renal Inj Prev. 2021; 10(3): e24. doi: 10.34172/jrip.2021.24.

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