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J Renal Inj Prev. 2017;6(2): 93-98.
doi: 10.15171/jrip.2017.18
PMID: 28497082
PMCID: PMC5423291
Scopus ID: 85031999297
  Abstract View: 6520
  PDF Download: 2970

Original Article

Evaluation of selenium on kidney function following ischemic injury in rats; protective effects and antioxidant activity

Amin Hasanvand 1, Abolfazl Abbaszadeh 2, Saeideh Darabi 3, Afshin Nazari 4, Mohammadreza Gholami 5, Ali Kharazmkia 6*

1 Department of Pharmacology, Faculty of pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran
3 azi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran; Lorestan Veterinary Organization Office, Khorramabad, Iran
4 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
5 Department of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran
6 Department of Pharmacotherapy, Faculty of pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
*Corresponding Author: *Corresponding author: Ali Kharazmkia, , Email: kharazmkia@gmail.com

Abstract

Introduction: Renal dysfunction is caused by ischemia-reperfusion (I/R) injury, which is a common problem in kidney surgery or kidney transplantation. The human body consists of enormous complex antioxidant systems, which inquires adequate selenium (Se) absorption for normal physiologic function. It is known that Se has some antioxidant effects.

Objectives: In the present research, effects of the Se on damages caused by I/R injury investigated.

Materials and Methods: In this experimental research, four groups of rats (weighing 220±10 g) used, include control group, I/R group, healthy group treated with Se for two weeks, and I/R group with two-week Se treatment. On the test day, I/R was treated in both right and left renal arteries for 45 minutes and the reperfusion was done for 24 hours.

Results: In I/R group, the amount of urea and serum creatinine (Cr) was an injury indicator of the kidney cells which showed a significant increase compared with the control group. When the treatment with Se significantly reduced these indicators, glutathione (GSH) enzyme levels reduced significantly in the second group and the enzyme levels increased due to Se treatment in the fourth group. Furthermore, malondialdehyde (MDA) enzyme levels increased in I/R group due to the Se treatment in the fourth group which was significantly reduced. In addition, the tissue damage was reduced in the fourth group compared with I/R group.

Conclusion: Se has a protective effect against the I/R injury. This effect might be due to the antioxidant properties of Se.


Implication for health policy/practice/research/medical education:

Ischemia-reperfusion injury is the major etiology of chronic renal failure. Its pathophysiology has been widely studied. Oxidative stress has been known to be involved in it extensively. Thus a couple of antioxidative substances has been studied to figure out if they can modify ischemia-reperfusion injury. In current study we tried to assess a couple of oxidative biomarkers in ischemia-reperfusion injury, following administration of Se to show whether it can amend their changes and reduce Cr and albumin/Cr as well.

Please cite this paper as: Hasanvand A, Abbaszadeh A, Darabi S, Nazari A, Gholami M, Kharazmkia A. Evaluation of selenium on kidney function following ischemic injury in rats; protective effects and antioxidant activity. J Renal Inj Prev. 2017;6(2):93-98. DOI: 10.15171/jrip.2017.18.

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