Abstract
Introduction: Glutathione (GSH) protects the tissue and cell from oxidative injury.
Objectives: In the current study, we investigated the possible effects of GSH on liver markers,
oxidative stress and inflammatory indices in rat with renal ischemia reperfusion (RIR) injury.
Materials and Methods: Twenty-four adult male Wistar rats were divided into 3 groups (n=8).
Group I (the control group), group II (the RIR group) received saline (0.25 mL/d, intraperitoneally;
i.p.), group III as the RIR group that received GSH (100 mg/kg/d, i.p.). The treatment with saline or
GSH began daily 14 days before RIR induction. RIR was induced by clamping renal pedicles for 45
minutes and 24 hours of reperfusion.
Results: RIR significantly increased the serum level of nitric oxide (NO), the serum activities
of aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyltransferase
(GGT), the serum and renal levels of malondialdehyde (MDA), and the serum activity of
myeloperoxidase (MPO). However, RIR significantly decreased the serum and renal levels of
GSH, serum paraoxonase 1 (PON1) activity, and the serum and renal activities of catalase (CAT)
and glutathione peroxidase (GPX). GSH administration could significantly improve the serum
activities of AST, GGT, MPO, GPX and PON1 and serum levels of NO, renal MDA, GSH levels, and
serum and also renal CAT activities.
Conclusion: Our study indicated that GSH administration ameliorated RIR injury in rats by
improving the activities of liver markers and antioxidant enzymes, the levels of MDA, NO, GSH
and MPO activity.